ARG66426
anti-IL33R / ST2 antibody [SQab18128]
anti-IL33R / ST2 antibody [SQab18128] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [SQab18128] recognizes IL33R / ST2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab18128 |
| Isotype | IgG1, kappa |
| Target Name | IL33R / ST2 |
| Antigen Species | Human |
| Immunogen | Recombinant Human IL33R / ST2 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | IL33R; DER4; ST2V; T1; Interleukin-1 receptor-like 1; FIT-1; ST2; ST2L; Protein ST2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | Affinity purification with immunogen. |
|---|---|
| Buffer | PBS (pH 7.4) and 0.01% Thimerosal. |
| Preservative | 0.01% Thimerosal |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL1RL1 |
| Gene Full Name | interleukin 1 receptor-like 1 |
| Background | The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| Function | Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. [UniProt] |
| Cellular Localization | Isoform C: Cell membrane. Isoform B: Secreted. Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 63 kDa |
Images (4) Click the Picture to Zoom In
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ARG66426 anti-IL33R / ST2 antibody [SQab18128] ICC/IF image
Immunofluorescence: HT29 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66426 anti-IL33R / ST2 antibody [SQab18128] (green) at 1:400 and cell nuclei were stained with Hoechst 33342 (blue).
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ARG66426 anti-IL33R / ST2 antibody [SQab18128] FACS image
Flow Cytometry: HT29 cells were stained with ARG66426 anti-IL33R / ST2 antibody [SQab18128] at 1 µg/ml (red) and without antibody control (black).
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ARG66426 anti-IL33R / ST2 antibody [SQab18128] WB image
Western blot: 50 µg of HT29 cell lysate were stained with ARG66426 anti-IL33R / ST2 antibody [SQab18128] at 1:2000 dilution.
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ARG66426 anti-IL33R / ST2 antibody [SQab18128] ELISA image
ELISA: Titration curve of ARG66426 anti-IL33R / ST2 antibody [SQab18128]. Red: ST2; Black: BSA (negative control).
