ARG66425

anti-IL33 antibody [SQab18127]

anti-IL33 antibody [SQab18127] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [SQab18127] recognizes IL33
Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone SQab18127
Isotype IgG2a, kappa
Target Name IL33
Antigen Species Human
Immunogen Recombinant Human IL33 protein.
Conjugation Un-conjugated
Alternate Names 95-270; NF-HEV; Interleukin-33; C9orf26; IL1F11; 99-270; Interleukin-1 family member 11; IL-33; IL-1F11; Nuclear factor from high endothelial venules; NFEHEV; DVS27; 109-270

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:3000 - 1:10000
FACS1:400 - 1:1000
ICC/IF1:400 - 1:1000
IHC-PAssay-dependent
WB1:2500 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4) and 0.01% Thimerosal.
Preservative 0.01% Thimerosal
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 90865 Human IL33

Swiss-port # O95760 Human Interleukin-33

Gene Symbol IL33
Gene Full Name interleukin 33
Background The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Function Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation. [UniProt]
Cellular Localization Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Note=Associates with heterochromatin and mitotic chromosomes (PubMed:17185418). [UniProt]
Calculated MW 31 kDa
PTM The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG66425 anti-IL33 antibody [SQab18127] ICC/IF image

    Immunofluorescence: HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66425 anti-IL33 antibody [SQab18127] (green) at 1:400 dilution and cell nuclei were stained with Hoechst 33342 (blue).

  • ARG66425 anti-IL33 antibody [SQab18127] FACS image

    Flow Cytometry: HeLa cells were stained with ARG66425 anti-IL33 antibody [SQab18127] at 1 µg/ml (red) and without antibody control (black).

  • ARG66425 anti-IL33 antibody [SQab18127] WB image

    Western blot: 50 ng of Recombinant IL33 protein stained with ARG66425 anti-IL33 antibody [SQab18127] at 1:5000 dilution.

  • ARG66425 anti-IL33 antibody [SQab18127] ELISA image

    ELISA: Titration curve of ARG66425 anti-IL33 antibody [SQab18127]. Red: IL33; Black: BSA (negative control).