ARG65985
anti-IL3 antibody [5A4-1-16]
anti-IL3 antibody [5A4-1-16] for ELISA,Neutralizing,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [5A4-1-16] recognizes IL3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, Neut, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5A4-1-16 |
| Isotype | IgG |
| Target Name | IL3 |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human IL3. (APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF) |
| Conjugation | Un-conjugated |
| Alternate Names | MCGF; Mast cell growth factor; P-cell-stimulating factor; Hematopoietic growth factor; IL-3; Interleukin-3; Multipotential colony-stimulating factor; MULTI-CSF |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL3 |
| Gene Full Name | interleukin 3 |
| Background | The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008] |
| Function | Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. [UniProt] |
| Calculated MW | 17 kDa |
Images (3) Click the Picture to Zoom In
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ARG65985 anti-IL3 antibody [5A4-1-16] WB image
Western blot: 250 - 0.24 ng (left to right) of Human IL-3 stained with ARG65985 anti-IL3 antibody [5A4-1-16], under non-reducing conditions.
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ARG65985 anti-IL3 antibody [5A4-1-16] standard curve image
Sandwich ELISA: ARG65985 anti-IL3 antibody [5A4-1-16] as a capture antibody at 4.0 - 8.0 µg/ml combined with ARG65999 anti-IL3 antibody (Biotin) as a detection antibody at ~ 0.50 - 1.0 µg/ml. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG65985 anti-IL3 antibody [5A4-1-16] WB image
Western blot: 250 - 0.24 ng (left to right) of Human IL-3 stained with ARG65985 anti-IL3 antibody [5A4-1-16], under reducing conditions.
