ARG56791

anti-IL21 antibody (Biotin)

anti-IL21 antibody (Biotin) for ELISA,Western blot and Mouse

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes IL21
Tested Reactivity Ms
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL21
Antigen Species Mouse
Immunogen E.coli derived Recombinant Mouse IL-21.
(MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS)
Conjugation Biotin
Alternate Names Za11; IL-21; CVID11; Interleukin-21

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56681 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 60505 Mouse IL21

Swiss-port # Q9ES17 Mouse Interleukin-21

Gene Symbol Il21
Gene Full Name interleukin 21
Background This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
Function Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. [UniProt]
Calculated MW 19 kDa

Images (2) Click the Picture to Zoom In

  • ARG56791 anti-IL21 antibody (Biotin) standard curve image

    Direct ELISA: ARG56791 anti-IL21 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56791 anti-IL21 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56791 anti-IL21 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56681 anti-IL21 antibody as a capture antibody. Results of a typical standard run with optical density.