ARG56659

anti-IL2 antibody

anti-IL2 antibody for ELISA,Neutralizing,Western blot and Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes IL2
Tested Reactivity Rat
Tested Application ELISA, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL2
Antigen Species Rat
Immunogen E.coli derived Recombinant Rat IL-2.
(MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG SENKFECQFD DEPATVVEFL RRWIAIAQSI ISTM)
Conjugation Un-conjugated
Alternate Names TCGF; IL-2; lymphokine; Interleukin-2; Aldesleukin; T-cell growth factor

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56769 as a detection antibody
Neut< 0.1 µg/ml (To yield [ND50] of the biological activity of Rat IL - 2 (1.0 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 116562 Rat IL2

Swiss-port # P17108 Rat Interleukin-2

Gene Symbol Il2
Gene Full Name interleukin 2
Background The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
Function Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProt]
Calculated MW 18 kDa

Images (3) Click the Picture to Zoom In

  • ARG56659 anti-IL2 antibody WB image

    Western blot: 250 - 0.24 ng of Rat IL-2 stained with ARG56659 anti-IL2 antibody, under reducing conditions.

  • ARG56659 anti-IL2 antibody WB image

    Western blot: 250 - 0.24 ng of Rat IL-2 stained with ARG56659 anti-IL2 antibody, under non-reducing conditions.

  • ARG56659 anti-IL2 antibody standard curve image

    Sandwich ELISA: ARG56659 anti-IL2 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56769 anti-IL2 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.