ARG56658

anti-IL2 antibody

anti-IL2 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human

Overview

Product Description Goat Polyclonal antibody recognizes IL2
Tested Reactivity Hu
Tested Application ELISA, IHC-P, Neut, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IL2
Antigen Species Human
Immunogen E.coli derived Recombinant Human IL-2.
(MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT)
Conjugation Un-conjugated
Alternate Names TCGF; IL-2; lymphokine; Interleukin-2; Aldesleukin; T-cell growth factor

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56767 as a detection antibody
IHC-P0.5 µg/ml
Neut0.12 - 0.16 µg/ml (To yield [ND50] of the biological activity of hIL - 2 (2.0 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3558 Human IL2

Swiss-port # P60568 Human Interleukin-2

Gene Symbol IL2
Gene Full Name interleukin 2
Background The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
Function Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProt]
Calculated MW 18 kDa

Images (5) Click the Picture to Zoom In

  • ARG56658 anti-IL2 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56658 anti-IL2 antibody concentration is 0.5 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56658 anti-IL2 antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-2 stained with ARG56658 anti-IL2 antibody, under reducing conditions.

  • ARG56658 anti-IL2 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56658 anti-IL2 antibody concentration is 0.5 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56658 anti-IL2 antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-2 stained with ARG56658 anti-IL2 antibody, under non-reducing conditions.

  • ARG56658 anti-IL2 antibody standard curve image

    Sandwich ELISA: ARG56658 anti-IL2 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56767 anti-IL2 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.