ARG10197

anti-IL2 antibody [9F9]

anti-IL2 antibody [9F9] for ELISA and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [9F9] recognizes Human IL-2
Tested Reactivity Hu
Tested Application ELISA
Host Mouse
Clonality Monoclonal
Clone 9F9
Isotype IgG2a, kappa
Target Name IL2
Antigen Species Human
Immunogen Recombinant human IL-2
Conjugation Un-conjugated
Alternate Names TCGF; IL-2; lymphokine; Interleukin-2; Aldesleukin; T-cell growth factor

Application Instructions

Application Note ELISA: mAb clone 9F9 can be used as capture antibody to detect recombinant human IL-2. This clone matches with HRP conjugated mAb clone 5A8, mAb clone 10C4 and clone 6B1.

When used reversely as detection antibody, 9F9 also matches with 5A8, 10C5 and 6B1. However, 9F9 produced better results when it was used as capture antibody.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3558 Human IL2

Swiss-port # P60568 Human Interleukin-2

Gene Symbol IL2
Gene Full Name interleukin 2
Background Interleukin 2 (IL-2) is the cytokine secreted by T-cells immediately after the T cell receptors are activated by the binding of foreign antigens. The cytokine stimulates the growth, proliferation and differentiation of naïve T-cells into “effecter T-cells”, promotes the survival of antigen specific “memory T-cell”, and stimulates the maturation of “regulatory T-cells”. The cytokine is crucial for the development of adapted immunity, immune memory and the modulation of auto-immunity.
Function Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProt]
Research Area Immune System antibody
Calculated MW 18 kDa