ARG56600
anti-IL2 antibody [2G7-25-3]
anti-IL2 antibody [2G7-25-3] for ELISA,Western blot and Rat
Overview
| Product Description | Mouse Monoclonal antibody [2G7-25-3] recognizes IL2 |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2G7-25-3 |
| Isotype | IgG1, kappa |
| Target Name | IL2 |
| Antigen Species | Rat |
| Immunogen | E.coli derived Recombinant Rat IL-2. (MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG SENKFECQFD DEPATVVEFL RRWIAIAQSI ISTM) |
| Conjugation | Un-conjugated |
| Alternate Names | TCGF; IL-2; lymphokine; Interleukin-2; Aldesleukin; T-cell growth factor |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Il2 |
| Gene Full Name | interleukin 2 |
| Background | The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008] |
| Function | Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProt] |
| Calculated MW | 18 kDa |
Images (3) Click the Picture to Zoom In
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ARG56600 anti-IL2 antibody [2G7-25-3] WB image
Western blot: 250 - 0.24 ng (left to right) of Rat IL-2 stained with ARG56600 anti-IL2 antibody [2G7-25-3], under reducing conditions.
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ARG56600 anti-IL2 antibody [2G7-25-3] WB image
Western blot: 250 - 0.24 ng (left to right) of Rat IL-2 stained with ARG56600 anti-IL2 antibody [2G7-25-3], under non-reducing conditions.
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ARG56600 anti-IL2 antibody [2G7-25-3] standard curve image
Sandwich ELISA: ARG56600 anti-IL2 antibody [2G7-25-3] as a capture antibody at 14.0 - 16.0 µg/ml combined with ARG56769 anti-IL2 antibody (Biotin) as a detection antibody at 2.0 - 4.0 µg/ml. Results of a typical standard run with optical density.
