ARG42445
anti-IL2 Receptor beta antibody [TU27] (low endotoxin)
anti-IL2 Receptor beta antibody [TU27] (low endotoxin) for Flow cytometry,Functional study,Immunoprecipitation and Human
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [TU27] recognizes IL2 Receptor beta |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, FuncSt, IP |
| Specificity | The mouse monoclonal antibody TU27 recognizes an extracellular epitope of CD122 (IL-2R beta), a 70-75 kDa type I transmembrane glycoprotein constitutively expressed by NK cells and a T cell subset, and upregulated upon activation. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TU27 |
| Isotype | IgG1, kappa |
| Target Name | IL2 Receptor beta |
| Antigen Species | Human |
| Immunogen | TL-Mor cell line. |
| Conjugation | Un-conjugated |
| Alternate Names | P70-75; IL-2RB; IL-2 receptor subunit beta; p75; Interleukin-2 receptor subunit beta; CD122; CD antigen CD122; High affinity IL-2 receptor subunit beta; IL-2R subunit beta; p70-75; IL15RB |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein. |
| Buffer | PBS |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P14784 Human Interleukin-2 receptor subunit beta |
|---|---|
| Gene Symbol | IL2RB |
| Gene Full Name | interleukin 2 receptor, beta |
| Background | The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. The use of alternative promoters results in multiple transcript variants encoding the same protein. The protein is primarily expressed in the hematopoietic system. The use by some variants of an alternate promoter in an upstream long terminal repeat (LTR) results in placenta-specific expression. [provided by RefSeq, Sep 2016] |
| Function | Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15 (PubMed:15123770). [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 61 kDa |
Images (3) Click the Picture to Zoom In
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ARG42445 anti-IL2 Receptor beta antibody [TU27] (low endotoxin) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG42445 anti-IL2 Receptor beta antibody [TU27] (low endotoxin) at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG42445 anti-IL2 Receptor beta antibody [TU27] (low endotoxin) FACS image
Flow Cytometry: Human CD3 negative lymphocytes stained with ARG42445 anti-IL2 Receptor beta antibody [TU27] (low endotoxin) at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody. Samples were co-stained with anti-CD56 antibody [LT56] (FITC) at 10 µl / 100 µl of peripheral whole blood.
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ARG42445 anti-IL2 Receptor beta antibody [TU27] (low endotoxin) FACS image
Flow Cytometry: Separation of Human CD122 positive CD56 positive CD3 negative lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG42445 anti-IL2 Receptor beta antibody [TU27] (low endotoxin) at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
