ARG41565

anti-IL18 antibody

anti-IL18 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes IL18
Tested Reactivity Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL18
Antigen Species Rat
Immunogen Recombinant protein corresponding to H37-S194 of Rat IL18.
Conjugation Un-conjugated
Alternate Names Iboctadekin; Interferon gamma-inducing factor; Interleukin-18; IL-18; IFN-gamma-inducing factor; IL-1 gamma; IGIF; IL-1g; Interleukin-1 gamma; IL1F4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 22 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16173 Mouse IL18

GeneID: 29197 Rat IL18

Swiss-port # P70380 Mouse Interleukin-18

Swiss-port # P97636 Rat Interleukin-18

Gene Symbol IL18
Gene Full Name interleukin 18
Background The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. [UniProt]
Cellular Localization Secreted. [UniProt]
Calculated MW 22 kDa
PTM The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG41565 anti-IL18 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat spleen tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41565 anti-IL18 antibody at 2 µg/ml dilution, overnight at 4°C.

  • ARG41565 anti-IL18 antibody WB image

    Western blot: 50 µg of samples under reducing conditions. Rat brain, Rat lung, Rat spleen, Rat liver, Rat testis, RH35 and PC-12 whole cell lysates stained with ARG41565 anti-IL18 antibody at 0.5 µg/ml dilution, overnight at 4°C.

  • ARG41565 anti-IL18 antibody WB image

    Western blot: 30 µg of sample under reducing conditions. Mouse spleen, Mouse thymus, Mouse lung and ANA-1 whole cell lysates stained with ARG41565 anti-IL18 antibody at 0.5 µg/ml dilution, overnight at 4°C.

  • ARG41565 anti-IL18 antibody FACS image

    Flow Cytometry: RH35 cells were blocked with 10% normal goat serum and then stained with ARG41565 anti-IL18 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.