ARG41564
anti-IL18 antibody
anti-IL18 antibody for Immunocytochemistry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IL18 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IL18 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to Y37-D193 of Human IL18. |
| Conjugation | Un-conjugated |
| Alternate Names | Iboctadekin; Interferon gamma-inducing factor; Interleukin-18; IL-18; IFN-gamma-inducing factor; IL-1 gamma; IGIF; IL-1g; Interleukin-1 gamma; IL1F4 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 22 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL18 |
| Gene Full Name | interleukin 18 |
| Background | The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011] |
| Function | Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 22 kDa |
| PTM | The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG41564 anti-IL18 antibody ICC image
Immunocytochemistry: Blood cells stained with ARG41564 anti-IL18 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41564 anti-IL18 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41564 anti-IL18 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41564 anti-IL18 antibody WB image
Western blot: 50 µg of sample under reducing conditions. HeLa whole cell lysate stained with ARG41564 anti-IL18 antibody at 0.5 µg/ml, overnight at 4°C.
