ARG66027

anti-IL17F antibody

anti-IL17F antibody for ELISA,Neutralizing,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes IL17F
Tested Reactivity Hu
Tested Application ELISA, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL17F
Antigen Species Human
Immunogen E. coli derived recombinant Human IL17F.
(MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ)
Conjugation Un-conjugated
Alternate Names Interleukin-17F; IL-17F; CANDF6; ML-1; ML1; Cytokine ML-1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66028 as a detection antibody
Neut0.9 - 1.2 µg/ml (To yield [ND50] of the biological activity of hIL-17F (50.0 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 112744 Human IL17F

Swiss-port # Q96PD4 Human Interleukin-17F

Gene Symbol IL17F
Gene Full Name interleukin 17F
Background The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008]
Function Stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. [UniProt]
Calculated MW 18 kDa

Images (3) Click the Picture to Zoom In

  • ARG66027 anti-IL17F antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-17F stained with ARG66027 anti-IL17F antibody, under non-reducing conditions.

  • ARG66027 anti-IL17F antibody standard curve image

    Sandwich ELISA: ARG66027 anti-IL17F antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66028 anti-IL17F antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66027 anti-IL17F antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-17F stained with ARG66027 anti-IL17F antibody, under reducing conditions.