ARG66025

anti-IL17D antibody

anti-IL17D antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes IL17D
Tested Reactivity Hu
Tested Application ELISA, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL17D
Antigen Species Human
Immunogen E. coli derived recombinant Human IL17D.
(APRAGRRPAR PRGCADRPEE LLEQLYGRLA AGVLSAFHHT LQLGPREQAR NASCPAGGRP ADRRFRPPTN LRSVSPWAYR ISYDPARYPR YLPEAYCLCR GCLTGLFGEE DVRFRSAPVY MPTVVLRRTP ACAGGRSVYT EAYVTIPVGC TCVPEPEKDA DSINSSIDKQ GAKLLLGPND APAGP)
Conjugation Un-conjugated
Alternate Names IL17D; Interleukin 17D; IL-17D; IL-27; Interleukin-17D; FLJ30846; IL27; Interleukin 27; Interleukin-27

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66026 as a detection antibody
IHC-P2.5 µg/ml
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 53342 Human IL17D

Swiss-port # Q8TAD2 Human Interleukin-17D

Gene Symbol IL17D
Gene Full Name interleukin 17D
Background The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17. The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent. [provided by RefSeq, Jul 2008]
Function Induces expression of IL-6, IL-8, and GM-CSF from endothelial cells. [UniProt]
Calculated MW 22 kDa

Images (4) Click the Picture to Zoom In

  • ARG66025 anti-IL17D antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal pancreas. The recommended ARG66025 anti-IL17D antibody concentration is 2.5 µg/ml for two hours at RT. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • ARG66025 anti-IL17D antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-17D stained with ARG66025 anti-IL17D antibody, under non-reducing conditions.

  • ARG66025 anti-IL17D antibody standard curve image

    Sandwich ELISA: ARG66025 anti-IL17D antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66026 anti-IL17D antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66025 anti-IL17D antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-17D stained with ARG66025 anti-IL17D antibody, under reducing conditions.