ARG22542

anti-IL10R subunit alpha antibody [1B1.3a]

anti-IL10R subunit alpha antibody [1B1.3a] for Flow cytometry and Mouse

Overview

Product Description Rat Monoclonal antibody [1B1.3a] recognizes IL10R subunit alpha
Tested Reactivity Ms
Species Does Not React With Hu
Tested Application FACS
Host Rat
Clonality Monoclonal
Clone 1B1.3a
Isotype IgG1
Target Name IL10R subunit alpha
Antigen Species Mouse
Immunogen Purified recombinant extracellular region of mouse CDw210a.
Conjugation Un-conjugated
Alternate Names IL-10R subunit alpha; CD210a; HIL-10R; Interleukin-10 receptor subunit alpha; IL-10R1; IL-10 receptor subunit alpha; IL10R; Interleukin-10 receptor subunit 1; IL-10R subunit 1; CDw210a; CDW210A; IL-10RA; CD antigen CD210; CD210

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:100
Application Note FACS: Due to low expression of CDw210a, sensitive staining techniques may be required. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16154 Mouse IL10RA

Swiss-port # Q61727 Mouse Interleukin-10 receptor subunit alpha

Gene Symbol Il10ra
Gene Full Name interleukin 10 receptor, alpha
Background The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]
Function Receptor for IL10; binds IL10 with a high affinity. [UniProt]
Calculated MW 63 kDa