ARG21419
anti-IL10 antibody [JES3-9D7]
anti-IL10 antibody [JES3-9D7] for ELISA,ELISPOT,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human,Rhesus Monkey,Virus
Publication1
Overview
| Product Description | Rat Monoclonal antibody [JES3-9D7] recognizes IL10 |
|---|---|
| Tested Reactivity | Hu, R. Mk, Virus |
| Tested Application | ELISA, ELISPOT, FACS, IHC-Fr, IHC-P, Neut, WB |
| Specificity | IL10 antibody clone JES3-9D7 reacts to Human/Viral/Rhesus/Cynomolgus/Sooty Mangabey IL-10, the antibody also reacts to vIL-10 from Epstein-Barr virus (EBV) BCRF1 gene. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | JES3-9D7 |
| Isotype | IgG1, kappa |
| Target Name | IL10 |
| Antigen Species | Human |
| Immunogen | COS-expressed human IL-10 |
| Conjugation | Un-conjugated |
| Alternate Names | IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10 |
Application Instructions
| Application Suggestion |
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| Application Note | ELISA: The clone JES3-9D7 may be paired with the purified clone JES3-12G8 in a sandwich ELISA. WB: PMID: 34551810 used this antibody to detect IL-10 in mouse samples by WB, but we have not tested it. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~17-18 kDa |
Properties
| Form | Liquid |
|---|---|
| Buffer | BBS (pH 8.2) |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL10 |
| Gene Full Name | interleukin 10 |
| Background | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011] |
| Function | Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt] |
| Calculated MW | 21 kDa |
Images (1) Click the Picture to Zoom In
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ARG21419 anti-IL10 antibody [JES3-9D7] standard curve image
ELISA: Human IL-10 detected by ARG21419 anti-IL10 antibody [JES3-9D7] as capture antibody ( ARG21420: Azide free version), and ARG21422 anti-IL10 antibody [JES3-12G8] (Biotin) as detection antibody, follow by incubation with streptavidin-HRP.
Specific References
Aging triggers an upregulation of a multitude of cytokines in the male and especially the female rodent hippocampus but more discrete changes in other brain regions
WB / Mouse
