ARG56766
anti-IL10 antibody (Biotin)
anti-IL10 antibody (Biotin) for ELISA,Western blot and Rat
Overview
| Product Description | Biotin-conjugated Rabbit Polyclonal antibody recognizes IL10 |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IL10 |
| Antigen Species | Rat |
| Immunogen | E.coli derived Recombinant Rat IL-10. (SKGHSIKGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNIVLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN) |
| Conjugation | Biotin |
| Alternate Names | IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Il10 |
| Gene Full Name | interleukin 10 |
| Background | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011] |
| Function | Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt] |
| Calculated MW | 21 kDa |
Images (4) Click the Picture to Zoom In
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ARG56766 anti-IL10 antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Rat IL-10 stained with ARG56766 anti-IL10 antibody (Biotin), under reducing conditions.
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ARG56766 anti-IL10 antibody (Biotin) standard curve image
Direct ELISA: ARG56766 anti-IL10 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.
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ARG56766 anti-IL10 antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Rat IL-10 stained with ARG56766 anti-IL10 antibody (Biotin), under non-reducing conditions.
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ARG56766 anti-IL10 antibody (Biotin) standard curve image
Sandwich ELISA: ARG56766 anti-IL10 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56656 anti-IL10 antibody as a capture antibody. Results of a typical standard run with optical density.
