ARG67118
anti-IL10 antibody
anti-IL10 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IL10 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IL10 |
| Antigen Species | Human |
| Immunogen | Human synthesized peptide derived IL10 |
| Conjugation | Un-conjugated |
| Alternate Names | IL10; Interleukin 10; IL-10; CSIF; Cytokine Synthesis Inhibitory Factor; IL10A; TGIF; T-Cell Growth Inhibitory Factor; Interleukin-10; GVHDS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Full Name | interleukin 10 |
| Background | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. |
| Function | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. |
| Cellular Localization | Secreted |
| Calculated MW | 21 kDa |
| PTM | Disulfide bond, Glycoprotein |
Images (4) Click the Picture to Zoom In
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ARG67118 anti-IL10 antibody IHC-P image
Immunohistochemistry: Human breast cancer stained with ARG67118 anti-IL10 antibody at 1:100l dilution.
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ARG67118 anti-IL10 antibody ICC/IF image
Immunofluorescence: A549 stained with ARG67118 anti-IL10 antibody at 1:200 dilution.
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ARG67118 anti-IL10 antibody WB image
Western blot: HepG2 stained with ARG67118 anti-IL10 antibody at 1:20000 dilution.
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ARG67118 anti-IL10 antibody WB image
Western blot: Mouse liver stained with ARG67118 anti-IL10 antibody at 1:20000 dilution.
