ARG56656

anti-IL10 antibody

anti-IL10 antibody for ELISA,Western blot and Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes IL10
Tested Reactivity Rat
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL10
Antigen Species Rat
Immunogen E.coli derived Recombinant Rat IL-10.
(SKGHSIKGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNIVLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN)
Conjugation Un-conjugated
Alternate Names IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56766 as a detection antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25325 Rat IL10

Swiss-port # P29456 Rat Interleukin-10

Gene Symbol Il10
Gene Full Name interleukin 10
Background The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt]
Calculated MW 21 kDa

Images (3) Click the Picture to Zoom In

  • ARG56656 anti-IL10 antibody WB image

    Western blot: 250 - 0.24 ng of Rat IL-10 stained with ARG56656 anti-IL10 antibody, under reducing conditions.

  • ARG56656 anti-IL10 antibody WB image

    Western blot: 250 - 0.24 ng of Rat IL-10 stained with ARG56656 anti-IL10 antibody, under non-reducing conditions.

  • ARG56656 anti-IL10 antibody standard curve image

    Sandwich ELISA: ARG56656 anti-IL10 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56766 anti-IL10 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.