ARG56655
anti-IL10 antibody
anti-IL10 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IL10 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, Neut, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IL10 |
| Antigen Species | Human |
| Immunogen | E.coli derived Recombinant Human IL-10. (MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N) |
| Conjugation | Un-conjugated |
| Alternate Names | IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL10 |
| Gene Full Name | interleukin 10 |
| Background | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011] |
| Function | Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt] |
| Calculated MW | 21 kDa |
Images (6) Click the Picture to Zoom In
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ARG56655 anti-IL10 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human pancreas adenocarcinoma tissue. The recommended ARG56655 anti-IL10 antibody concentration is 5.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56655 anti-IL10 antibody WB image
Western blot: 250 - 0.24 ng of Human IL-10 stained with ARG56655 anti-IL10 antibody, under reducing conditions.
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ARG56655 anti-IL10 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human pancreas adenocarcinoma tissue. The recommended ARG56655 anti-IL10 antibody concentration is 5.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56655 anti-IL10 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human pancreas adenocarcinoma tissue. The recommended ARG56655 anti-IL10 antibody concentration is 5.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56655 anti-IL10 antibody WB image
Western blot: 250 - 0.24 ng of Human IL-10 stained with ARG56655 anti-IL10 antibody, under non-reducing conditions.
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ARG56655 anti-IL10 antibody standard curve image
Sandwich ELISA: ARG56655 anti-IL10 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56765 anti-IL10 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
