ARG56645

anti-IL1 beta antibody

anti-IL1 beta antibody for ELISA,Neutralizing,Western blot and Human,Rat

Pyroptosis Study antibody

Overview

Product Description Goat Polyclonal antibody recognizes IL1 beta
Tested Reactivity Hu, Rat
Tested Application ELISA, Neut, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IL1 beta
Antigen Species Human
Immunogen E.coli derived Recombinant Human IL-1β.
(APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS)
Conjugation Un-conjugated
Alternate Names Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56755 as a detection antibody
Neut0.45 - 0.65 µg/ml (To yield [ND50] of the biological activity of hIL - 1β (5pg/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3553 Human IL1B

Swiss-port # P01584 Human Interleukin-1 beta

Gene Symbol IL1B
Gene Full Name interleukin 1, beta
Background IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Function IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]
Highlight Related products:
IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins; Anti-Goat IgG secondary antibodies;
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Research Area Pyroptosis Study antibody
Calculated MW 31 kDa
PTM Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Images (3) Click the Picture to Zoom In

  • ARG56645 anti-IL1 beta antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-1β stained with ARG56645 anti-IL1 beta antibody, under reducing conditions.

  • ARG56645 anti-IL1 beta antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-1β stained with ARG56645 anti-IL1 beta antibody, under non-reducing conditions.

  • ARG56645 anti-IL1 beta antibody standard curve image

    Sandwich ELISA: ARG56645 anti-IL1 beta antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56755 anti-IL1 beta antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.