ARG56777

anti-IL1 alpha antibody (Biotin)

anti-IL1 alpha antibody (Biotin) for ELISA,Western blot and Mouse

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes IL1 alpha
Tested Reactivity Ms
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL1 alpha
Antigen Species Mouse
Immunogen E.coli derived Recombinant Mouse IL-1α.
(SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS)
Conjugation Biotin
Alternate Names IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56667 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16175 Mouse IL1A

Swiss-port # P01582 Mouse Interleukin-1 alpha

Gene Symbol Il1a
Gene Full Name interleukin 1 alpha
Background The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
Highlight Related products:
IL1 alpha antibodies; IL1 alpha ELISA Kits; IL1 alpha recombinant proteins; Anti-Rabbit IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Calculated MW 31 kDa

Images (4) Click the Picture to Zoom In

  • ARG56777 anti-IL1 alpha antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Mouse IL-1α stained with ARG56777 anti-IL1 alpha antibody (Biotin), under reducing conditions.

  • ARG56777 anti-IL1 alpha antibody (Biotin) standard curve image

    Direct ELISA: ARG56777 anti-IL1 alpha antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56777 anti-IL1 alpha antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Mouse IL-1α stained with ARG56777 anti-IL1 alpha antibody (Biotin), under non-reducing conditions.

  • ARG56777 anti-IL1 alpha antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56777 anti-IL1 alpha antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56667 anti-IL1 alpha antibody as a capture antibody. Results of a typical standard run with optical density.