ARG55667

anti-IKB alpha antibody

Datasheet

Ordering Information

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Price (USD)

100 μl

389.00

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Datasheet

Overview

Product Description	Mouse Monoclonal antibody recognizes IKB alpha
Tested Reactivity	Hu, Ms
Tested Application	WB
Host	Mouse
Clonality	Monoclonal
Clone	1121CT8.6.1
Isotype	IgG1, kappa
Target Name	IKB alpha
Antigen Species	Human
Immunogen	Purified His-tagged Human IKB alpha
Conjugation	Un-conjugated
Alternate Names	NFKBI; I-kappa-B-alpha; IkB-alpha; NF-kappa-B inhibitor alpha; Major histocompatibility complex enhancer-binding protein MAD3; IKBA; IkappaBalpha; MAD-3

Application Instructions

Application Suggestion

Tested Application	Dilution
WB	1:1000

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control

NIH/3T3

Properties

Form	Liquid
Purification	Purification with Protein G.
Buffer	PBS and 0.09% (W/V) Sodium azide.
Preservative	0.09% (W/V) Sodium azide.
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 18035 Mouse NFKBIA GeneID: 4792 Human NFKBIA Swiss-port # P25963 Human NF-kappa-B inhibitor alpha Swiss-port # Q9Z1E3 Mouse NF-kappa-B inhibitor alpha
Gene Symbol	NFKBIA
Gene Full Name	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Background	This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
Function	Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. [UniProt]
Cellular Localization	Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Research Area	Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; IκB alpha degradation Study antibody; NF-kB Activation Study antibody
Calculated MW	36 kDa
PTM	Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation. Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability. Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36. Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.