ARG64539
anti-IGFBP6 antibody
anti-IGFBP6 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes IGFBP6 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IGFBP6 |
| Antigen Species | Human |
| Immunogen | C-RPQDVNRRDQQRNP |
| Conjugation | Un-conjugated |
| Alternate Names | IBP-6; IGF-binding protein 6; IGFBP-6; IBP6; Insulin-like growth factor-binding protein 6 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P24592 Human Insulin-like growth factor-binding protein 6 |
|---|---|
| Gene Symbol | IGFBP6 |
| Gene Full Name | insulin-like growth factor binding protein 6 |
| Function | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 25 kDa |
| PTM | O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid. |
Images (3) Click the Picture to Zoom In
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ARG64539 anti-IGFBP6 antibody WB image
Western Blot: Human Adrenal Gland lysate (35 µg protein in RIPA buffer) stained with ARG64539 anti-IGFBP6 antibody at 0.5 µg/ml dilution.
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ARG64539 anti-IGFBP6 antibody WB image
Western Blot: 1). Mock transfection; 2) IGFBP6 (RC204060) expressing plasmid transfected HEK293 cell lysate standed with ARG64539 anti-IGFBP6 antibody
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ARG64539 anti-IGFBP6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64539 anti-IGFBP6 antibody at 5 µg/ml dilution followed by AP-staining.
