ARG66585
anti-IGFBP3 antibody
anti-IGFBP3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IGFBP3 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Bov, Pig |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IGFBP3 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide within the center region of Human IGFBP3. |
| Conjugation | Un-conjugated |
| Alternate Names | IBP-3; IBP3; Insulin-like growth factor-binding protein 3; IGFBP-3; IGF-binding protein 3; BP-53 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 42 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P17936 Human Insulin-like growth factor-binding protein 3 Swiss-port # P47878 Mouse Insulin-like growth factor-binding protein 3 |
|---|---|
| Gene Symbol | IGFBP3 |
| Gene Full Name | insulin-like growth factor binding protein 3 |
| Background | This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 32 kDa |
| PTM | Phosphorylated by FAM20C in the extracellular medium. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66585 anti-IGFBP3 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was stained with ARG66585 anti-IGFBP3 antibody at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
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ARG66585 anti-IGFBP3 antibody WB image
Western blot: HuvEc, Raw264.7 and Mouse brain lysates stained with ARG66585 anti-IGFBP3 antibody.
