ARG64343
anti-IGFBP3 antibody
anti-IGFBP3 antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes IGFBP3 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IGFBP3 |
| Antigen Species | Human |
| Immunogen | C-RYKVDYESQSTDTQN |
| Conjugation | Un-conjugated |
| Alternate Names | IBP-3; IBP3; Insulin-like growth factor-binding protein 3; IGFBP-3; IGF-binding protein 3; BP-53 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P17936 Human Insulin-like growth factor-binding protein 3 |
|---|---|
| Background | This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 32 kDa |
| PTM | Phosphorylated by FAM20C in the extracellular medium. |
Images (1) Click the Picture to Zoom In
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ARG64343 anti-IGFBP3 antibody WB image
Western Blot: Human Breast cancer lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. stained with ARG64343 anti-IGFBP3 antibody at 0.02 µg/ml dilution.
