ARG40987
anti-IGFBP3 antibody
anti-IGFBP3 antibody for Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IGFBP3 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Hm |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IGFBP3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 182-198 of Human IGFBP3 (IKKGHAKDSQRYKVDYE). |
| Conjugation | Un-conjugated |
| Alternate Names | IBP-3; IBP3; Insulin-like growth factor-binding protein 3; IGFBP-3; IGF-binding protein 3; BP-53 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P15473 Rat Insulin-like growth factor-binding protein 3 Swiss-port # P17936 Human Insulin-like growth factor-binding protein 3 |
|---|---|
| Gene Symbol | IGFBP3 |
| Gene Full Name | insulin-like growth factor binding protein 3 |
| Background | This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 32 kDa |
| PTM | Phosphorylated by FAM20C in the extracellular medium. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG40987 anti-IGFBP3 antibody WB image
Western blot: 40 µg of 293T, MCF7, A549 and SW620 whole cell lysates stained with ARG40987 anti-IGFBP3 antibody at 0.5 µg/ml dilution.
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ARG40987 anti-IGFBP3 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Rat liver, Rat kidney and Rat heart lysates stained with ARG40987 anti-IGFBP3 antibody at 0.5 µg/ml dilution, overnight at 4°C.
