ARG56775

anti-IGF1 antibody (Biotin)

anti-IGF1 antibody (Biotin) for ELISA,Western blot and Mouse

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes IGF1
Tested Reactivity Ms
Tested Application ELISA, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IGF1
Antigen Species Mouse
Immunogen E.coli derived Recombinant Mouse IGF1.
(GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA)
Conjugation Biotin
Alternate Names MGF; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C; IGFI; IGF-I

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56665 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16000 Mouse IGF1

Swiss-port # P05017 Mouse Insulin-like growth factor I

Gene Symbol Igf1
Gene Full Name insulin-like growth factor 1
Background The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]
Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. [UniProt]
Calculated MW 22 kDa

Images (2) Click the Picture to Zoom In

  • ARG56775 anti-IGF1 antibody (Biotin) standard curve image

    Direct ELISA: ARG56775 anti-IGF1 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56775 anti-IGF1 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56775 anti-IGF1 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56665 anti-IGF1 antibody as a capture antibody. Results of a typical standard run with optical density.