ARG44440

anti-IFT52 antibody

anti-IFT52 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes IFT52
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name IFT52
Antigen Species Human
Immunogen Human IFT52 recombinant protein
Conjugation Un-conjugated
Alternate Names IFT52; Intraflagellar Transport 52; NGD5; C20orf9; CGI-53; NGD2; Intraflagellar Transport Protein 52 Homolog; Protein NGD5 Homolog

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/1x10^6 cells
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 51098 Human IFT52

Swiss-port # Q9Y366 Human Intraflagellar transport protein 52 homolog

Gene Symbol IFT52
Gene Full Name Intraflagellar Transport 52
Background This gene encodes a conserved proline-rich protein that is a component of the intraflagellar transport-B (IFT-B) core complex. The encoded protein is essential for the integrity of the IFT-B core complex, and for biosynthesis and maintenance of cilia. Mutations in this gene are associated with ciliopathy that affects the skeleton.
Function Involved in ciliogenesis as part of a complex involved in intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia.
Cellular Localization Cell projection, Cilium
Calculated MW 50 kDa

Images (8) Click the Picture to Zoom In

  • ARG44440 anti-IFT52 antibody IHC-P image

    Immunohistochemistry: Human colorectal adenocarcinoma stained with ARG44440 anti-IFT52 antibody at 2 μg/mL dilution.

  • ARG44440 anti-IFT52 antibody IHC-P image

    Immunohistochemistry: Human intestinal cancer stained with ARG44440 anti-IFT52 antibody at 5 μg/mL dilution.

  • ARG44440 anti-IFT52 antibody WB image

    Western blot: SH-SY5Y stained with ARG44440 anti-IFT52 antibody at 0.5 μg/mL dilution.

  • ARG44440 anti-IFT52 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG44440 anti-IFT52 antibody at 1 μg/10^6 cells dilution.

  • ARG44440 anti-IFT52 antibody IHC-P image

    Immunohistochemistry: Rat testis stained with ARG44440 anti-IFT52 antibody at 2 μg/mL dilution.

  • ARG44440 anti-IFT52 antibody WB image

    Western blot: Rat testis stained with ARG44440 anti-IFT52 antibody at 0.5 μg/mL dilution.

  • ARG44440 anti-IFT52 antibody IHC-P image

    Immunohistochemistry: Mouse testis stained with ARG44440 anti-IFT52 antibody at 2 μg/mL dilution.

  • ARG44440 anti-IFT52 antibody WB image

    Western blot: Mouse testis stained with ARG44440 anti-IFT52 antibody at 0.5 μg/mL dilution.