ARG52325
anti-IFNAR1 phospho (Ser535 / Ser539) antibody
anti-IFNAR1 phospho (Ser535 / Ser539) antibody for Immunohistochemistry,Western blot and Human,Rat
Cancer antibody; Immune System antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IFNAR1 phospho (Ser535 / Ser539) |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms, Bov, Dog, NHuPrm, Sheep |
| Tested Application | IHC, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IFNAR1 |
| Antigen Species | Human |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; IFRC; IFNAR; AVP; IFN-alpha/beta receptor 1; CRF2-1; IFN-R-1; Type I interferon receptor 1; IFNBR; Interferon alpha/beta receptor 1; IFN-alpha-REC |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for IFNAR1 protein phosphorylated at Ser535,539. Note: the molecular weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110 - 130k. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IFNAR1 |
| Gene Full Name | interferon (alpha, beta and omega) receptor 1 |
| Background | Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003). |
| Research Area | Cancer antibody; Immune System antibody |
| Calculated MW | 64 kDa |
| PTM | Ubiquitinated, leading to its internalization and degradation (PubMed:14532120, PubMed:15337770). Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation (PubMed:18056411). The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex (PubMed:24075985). Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination (PubMed:14532120, PubMed:15337770, PubMed:24075985). Phosphorylated on tyrosine residues by TYK2 tyrosine kinase (PubMed:7526154). Phosphorylated on tyrosine residues in response to interferon (PubMed:10049744). Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2. |
Images (1) Click the Picture to Zoom In
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ARG52325 anti-IFNAR1 phospho (Ser535 / Ser539) antibody WB image
Western blot: Immunoprecipitates from HEK 293 cells transfected with
1. Mock
2. IFNAR1 WT and
3. IFNAR1 Ser 535 Arg and Ser 539 Arg mutants stained with ARG52325 anti-IFNAR1 phospho (Ser535 / Ser539) antibody.
The immunolabeling is absent in the IFNAR1 Ser 535 and Ser 539 mutants. The labeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the dephosphopeptide (Dephos).Iimmunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT, and 3. IFNAR1 S535A and S539A mutants showing specific immunolabeling of the ~110k to ~130k IFNAR1 WT. The immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (Control). The immunolabeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).
