ARG66112
anti-IFN lambda 2 antibody
anti-IFN lambda 2 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IFN lambda 2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IFN lambda 2 |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human IFN lambda 2. (PVARLHGALP DARGCHIAQF KSLSPQELQA FKRAKDALEE SLLLKDCRCH SRLFPRTWDL RQLQVRERPM ALEAELALTL KVLEATADTD PALVDVLDQP LHTLHHILSQ FRACIQPQPT AGPRTRGRLH HWLYRLQEAP KKESPGCLEA SVTFNLFRLL TRDLNCVASG DLCV) |
| Conjugation | Un-conjugated |
| Alternate Names | Interleukin-28A; IFN-lambda-2; Interferon lambda-2; IL28A; IL-28A; Cytokine Zcyto20 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IFNL2 |
| Gene Full Name | interferon, lambda 2 |
| Background | This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008] |
| Function | Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression. [UniProt] |
| Calculated MW | 22 kDa |
Images (4) Click the Picture to Zoom In
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ARG66112 anti-IFN lambda 2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of normal Human skin. The recommended ARG66112 anti-IFN lambda 2 antibody concentration is 1.0 µg/ml overnight at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen. Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min.
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ARG66112 anti-IFN lambda 2 antibody WB image
Western blot: 250 - 0.24 ng of Human IFN-λ2 stained with ARG66112 anti-IFN lambda 2 antibody, under non-reducing conditions.
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ARG66112 anti-IFN lambda 2 antibody standard curve image
Sandwich ELISA: ARG66112 anti-IFN lambda 2 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66113 anti-IFN lambda 2 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66112 anti-IFN lambda 2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of normal Human skin. The recommended ARG66112 anti-IFN lambda 2 antibody concentration is 1.0 µg/ml overnight at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen. Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min.
