ARG43398
anti-IFN gamma antibody (Biotin)
anti-IFN gamma antibody (Biotin) for ELISA,Western blot and Mouse
Overview
| Product Description | Biotin-conjugated Rabbit Polyclonal antibody recognizes IFN gamma |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IFN gamma |
| Antigen Species | Mouse |
| Immunogen | E. coli-derived recombinant Mouse IFN gamma. |
| Conjugation | Biotin |
| Alternate Names | IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | Sandwich ELISA (Capture antibody - Detection antibody): ARG56459 - ARG43398 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IFNG |
|---|---|
| Gene Full Name | interferon, gamma |
| Background | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015] |
| Function | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 19 kDa |
| PTM | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG43398 anti-IFN gamma antibody (Biotin) WB image
Western blot: 3.0 - 1.5 ng (left to right) of Recombinant Murine IFN gamma protein stained with ARG43398 anti-IFN gamma antibody (Biotin), under reducing conditions.
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ARG43398 anti-IFN gamma antibody (Biotin) standard curve image
Sandwich ELISA: ARG43398 anti-IFN gamma antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56459 anti-IFN gamma antibody as a capture antibody. Results of a typical standard run with optical density reading at 450 - 620 nm.
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ARG43398 anti-IFN gamma antibody (Biotin) WB image
Western blot: 3.0 - 1.5 ng (left to right) of Recombinant Murine IFN gamma protein stained with ARG43398 anti-IFN gamma antibody (Biotin), under non-reducing conditions.
