ARG56606
anti-IFN gamma antibody
anti-IFN gamma antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IFN gamma |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, Neut, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IFN gamma |
| Antigen Species | Human |
| Immunogen | E.coli derived Recombinant Human IFN-γ. (MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ) |
| Conjugation | Un-conjugated |
| Alternate Names | IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | IFNG |
| Gene Full Name | interferon, gamma |
| Background | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015] |
| Function | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt] |
| Highlight | Related products: IFN gamma antibodies; IFN gamma ELISA Kits; IFN gamma Duos / Panels; IFN gamma recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines |
| Calculated MW | 19 kDa |
| PTM | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. |
Images (4) Click the Picture to Zoom In
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ARG56606 anti-IFN gamma antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56606 anti-IFN gamma antibody concentration is 0.5 - 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56606 anti-IFN gamma antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56606 anti-IFN gamma antibody concentration is 0.5 - 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56606 anti-IFN gamma antibody WB image
Western blot: 250 - 0.24 ng of Human IFN-γ stained with ARG56606 anti-IFN gamma antibody, under non-reducing conditions.
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ARG56606 anti-IFN gamma antibody standard curve image
Sandwich ELISA: ARG56606 anti-IFN gamma antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with anti-IFN-gamma antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
