ARG65395

anti-IFN gamma antibody [4S.B3]

anti-IFN gamma antibody [4S.B3] for CyTOF®-candidate,ELISA,Flow cytometry,Functional study,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Radioimmunoassay,Western blot and Human,Primates

Cancer antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [4S.B3] recognizes IFN gamma
Tested Reactivity Hu, NHuPrm
Tested Application CyTOF®-candidate, ELISA, FACS, FuncSt, ICC/IF, IHC-P, IP, RIA, WB
Specificity The clone 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
Host Mouse
Clonality Monoclonal
Clone 4S.B3
Isotype IgG1
Target Name IFN gamma
Antigen Species Human
Immunogen Interferon gamma derived from human leukocytes
Conjugation Un-conjugated
Alternate Names IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
ELISAAssay-dependent
FACS1 - 4 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
RIAAssay-dependent
WB1:100 - 1:200
Application Note ELISA: Used as detection antibody in combination with capture antibody NIB42
Functional study: Neutralization (weak).
FACS: Intracellular staining, permeabilization is needed.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~16-18 kDa (monomer); 34-36 kDa (dimer)

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3458 Human IFNG

Swiss-port # P01579 Human Interferon gamma

Background The Interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN.
IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt]
Highlight Related products:
IFN gamma antibodies; IFN gamma ELISA Kits; IFN gamma Duos / Panels; IFN gamma recombinant proteins; Anti-Mouse IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
CyTOF-candidate Antibodies
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 19 kDa
PTM Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.