ARG59751
anti-IDH3B antibody
anti-IDH3B antibody for Western blot and Human,Mouse,Rat,Pig
Overview
| Product Description | Goat Polyclonal antibody recognizes IDH3B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IDH3B |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 33-46 of Human IDH3B. (NP_008830.2; NP_777280.1. C-HAASRSQAEDVRVE) |
| Conjugation | Un-conjugated |
| Alternate Names | RP46; Isocitric dehydrogenase subunit beta; NAD; Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial; H-IDHB; EC 1.1.1.41; + |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 40 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IDH3B |
| Gene Full Name | isocitrate dehydrogenase 3 (NAD+) beta |
| Background | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 42 kDa |
Images (3) Click the Picture to Zoom In
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ARG59751 anti-IDH3B antibody WB image
Western blot: 35 µg of Human skeletal muscle lysate (in RIPA buffer) stained with ARG59751 anti-IDH3B antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG59751 anti-IDH3B antibody WB image
Western blot: 35 µg of Mouse heart and Mouse muscle lysate (in RIPA buffer) stained with ARG59751 anti-IDH3B antibody at 2 µg/ml dilution and incubated at RT for 1 hour.
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ARG59751 anti-IDH3B antibody WB image
Western blot: 35 µg of Pig heart lysate (in RIPA buffer) stained with ARG59751 anti-IDH3B antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
