ARG65798
anti-IDH2 antibody
anti-IDH2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig
Overview
| Product Description | Goat Polyclonal antibody recognizes IDH2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IDH2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human IDH2. (CIHGLSNVKLNE) |
| Conjugation | Un-conjugated |
| Alternate Names | D2HGA2; IDH; Isocitrate dehydrogenase [NADP], mitochondrial; IDPM; EC 1.1.1.42; mNADP-IDH; ICD-M; IDP; IDHM; NADP; Oxalosuccinate decarboxylase |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IDH2 |
| Gene Full Name | isocitrate dehydrogenase 2 (NADP+), mitochondrial |
| Background | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| Function | Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. [UniProt] |
| Calculated MW | 51 kDa |
| PTM | Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3. |
Images (3) Click the Picture to Zoom In
-
ARG65798 anti-IDH2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65798 anti-IDH2 antibody at 3.75 µg/ml dilution followed by AP-staining.
-
ARG65798 anti-IDH2 antibody WB image
Western blot: 35 µg of Human Heart lysate stained with ARG65798 anti-IDH2 antibody at 0.1 µg/ml dilution (1 hour incubation).
-
ARG65798 anti-IDH2 antibody WB image
Western blot: 35 µg of A) Mouse, B) Rat, and C) Pig heart lysates stained with ARG65798 anti-IDH2 antibody at 0.1 µg/ml dilution (1 hour incubation).
