Overview

Product Description	Rabbit Polyclonal antibody recognizes ID2
Tested Reactivity	Hu, Ms, Rat
Tested Application	IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	ID2
Antigen Species	Human
Immunogen	Recombinant fusion protein corresponding to aa. 75-134 of Human ID2 (NP_002157.2).
Conjugation	Un-conjugated
Alternate Names	ID2H; DNA-binding protein inhibitor ID-2; GIG8; ID2A; Class B basic helix-loop-helix protein 26; Inhibitor of DNA binding 2; bHLHb26; Inhibitor of differentiation 2

Application Instructions

Application Suggestion	Tested Application Dilution IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	293T
Observed Size	16 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 15902 Mouse ID2 GeneID: 25587 Rat ID2 GeneID: 3398 Human ID2 Swiss-port # P41136 Mouse DNA-binding protein inhibitor ID-2 Swiss-port # P41137 Rat DNA-binding protein inhibitor ID-2 Swiss-port # Q02363 Human DNA-binding protein inhibitor ID-2 More
Gene Symbol	ID2
Gene Full Name	inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
Background	The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq, Aug 2011]
Function	Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism. [UniProt]
Cellular Localization	Cytoplasm, Nucleus. [UniProt]
Calculated MW	15 kDa

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