ARG54401

anti-ICAD antibody

anti-ICAD antibody for ICC/IF,Western blot and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ICAD
Tested Reactivity Hu
Tested Application ICC/IF, WB
Specificity This antibody recognizes non-cleaved (45kDa) and cleaved DFF45.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ICAD
Antigen Species Human
Immunogen Peptide corresponding to aa 313-331 at the C-terminus of human DFF45 (accession no. NP_004392).
Conjugation Un-conjugated
Alternate Names DFF-45; DNA fragmentation factor 45 kDa subunit; Inhibitor of CAD; ICAD; DFF1; DNA fragmentation factor subunit alpha

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa, Jurkat, A431 and K562

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1676 Human DFFA

Swiss-port # O00273 Human DNA fragmentation factor subunit alpha

Gene Symbol DFFA
Gene Full Name DNA fragmentation factor, 45kDa, alpha polypeptide
Background A human DNA fragmentation factor (DFF) which is cleaved by caspase-3 during apoptosis was identified recently. The mouse homologue of human DFF was identified as a DNase inhibitor and was designated ICAD. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in nuclei. Cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is a hallmark of apoptotic cell death.
Function Inhibitor of the caspase-activated DNase (DFF40). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
Calculated MW 37 kDa
PTM Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.

Images (2) Click the Picture to Zoom In

  • ARG54401 anti-ICAD antibody WB image

    Western blot: H:HeLaJ:Jurkat; A:A431; K:K562 stained with ARG54401 anti-ICAD antibody at 1 μg/ml dilution.

  • ARG54401 anti-ICAD antibody ICC/IF image

    Immunofluorescence: HeLa stained with ARG54401 anti-ICAD antibody at 5 μg/ml dilution.