ARG64670
anti-IA2 antibody
anti-IA2 antibody for Western blot and Mouse
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes IA2 |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Hu, Rat |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IA2 |
| Antigen Species | Human |
| Immunogen | C-QGEGNIKKNRHPD |
| Conjugation | Un-conjugated |
| Alternate Names | Islet cell antigen 512; ICA 512; ICA512; IA-2/PTP; IA2; PTP IA-2; Receptor-type tyrosine-protein phosphatase-like N; Islet cell autoantigen 3; IA-2; R-PTP-N |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single catalytic domain, and thus represents a receptor-type PTP. This PTP was found to be an autoantigen that is reactive with insulin-dependent diabetes mellitus (IDDM) patient sera, and thus may be a potential target of autoimmunity in diabetes mellitus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010] |
|---|---|
| Research Area | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 106 kDa |
| PTM | N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. Subject to proteolytic cleavage at multiple sites (PubMed:11483505). Subject to cleavage on a pair of basic residues (By similarity). On exocytosis of secretory granules in pancreatic beta-cells ICA512-TMF is transiently inserted in the plasma-membrane and cleaved by mu-type calpain CPN1 to yield ICA512-CCF (By similarity). Sumoylated at two sites including Lys-754. Sumoylation decreases interaction with STAT5. |
Images (1) Click the Picture to Zoom In
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ARG64670 anti-IA2 antibody WB image
Western Blot: NIH 3T3 lysate (35 µg protein in RIPA buffer) stained with ARG64670 anti-IA2 antibody at 0.5 µg/ml dilution.
