ARG53946
anti-Hsp 90 antibody [MBH90AB]
anti-Hsp 90 antibody [MBH90AB] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Bovine
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [MBH90AB] recognizes Hsp 90 |
|---|---|
| Tested Reactivity | Hu, Ms, Bov |
| Tested Application | IHC-P, WB |
| Specificity | The clone MBH90AB recognizes the epitope EEEVE within N-terminal part of ubiquitously expressed Hsp90 alpha and Hsp90 beta proteins with calculated MW of 84.7 kDa and 83.3 kDa, respectively, however, migrating as 90 kDa bands under reducing SDS-PAGE conditions. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MBH90AB |
| Isotype | IgG1 |
| Target Name | Hsp 90 |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to the sequence EEVHHGEEEVEC within N-terminal part of human Hsp90. |
| Conjugation | Un-conjugated |
| Alternate Names | EL52; Hsp90; HSPC1; Heat shock 86 kDa; LAP-2; HSP90N; LAP2; HSP90A; HSPCAL4; HSP89A; HSP86; HSP 86; HSPCA; Lipopolysaccharide-associated protein 2; HSPCAL1; LPS-associated protein 2; HSPN; Renal carcinoma antigen NY-REN-38; Heat shock protein HSP 90-alpha; Hsp89 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSP90AA1 |
| Gene Full Name | heat shock protein 90kDa alpha (cytosolic), class A member 1 |
| Background | Hsp90 (heat shock protein 90) is one of the most abundant chaperones in the cytosol of eukaryotic cells. It interacts with various proteins, including protein kinases and transcription factors, and either facilitates their stabilization and activation or directs them for proteasomal degradation. Hsp90 thus affects multiple signaling pathways and biological processes and modulation of this single target offers the prospect of simultaneous intervence to various key points of oncogenic transformation. Hsp90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis. There are two isoforms, alpha and beta, of vertebrate Hsp90. Whereas Hsp90 beta is expressed constitutively to a high level, Hsp90 alpha is stress-inducible and is overexpressed in many cancerous cells._x000D_ |
| Function | Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. [UniProt] |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 85 kDa |
| PTM | ISGylated. S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. |
Images (2) Click the Picture to Zoom In
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ARG53946 anti-Hsp 90 antibody [MBH90AB] IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue stained with ARG53946 anti-Hsp 90 antibody [MBH90AB].
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ARG53946 anti-Hsp 90 antibody [MBH90AB] WB image
Western blot: 1. Purified Hsp90 alpha protein, 2. Hsp90 beta protein, 3. MCF-7 cell lysate, 4. MCF-7-UV cell lysate stained with ARG53946 anti-Hsp 90 antibody [MBH90AB].
