ARG54450
anti-Hsp 90 alpha antibody [D7 alpha (D7a)]
anti-Hsp 90 alpha antibody [D7 alpha (D7a)] for Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Chicken,Pig,Rabbit
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [D7 alpha (D7a)] recognizes Hsp 90 alpha |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Chk, Pig, Rb |
| Tested Application | IP, WB |
| Specificity | This antibody recognizes human, mouse, rat, rabbit, bovine, porcine, and chicken Hsp90α (90 kDa). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | D7 alpha (D7a) |
| Isotype | IgG1 |
| Target Name | Hsp 90 alpha |
| Antigen Species | Chicken |
| Immunogen | Full-length Hsp90 purified from chicken brain |
| Conjugation | Un-conjugated |
| Alternate Names | EL52; Hsp90; HSPC1; Heat shock 86 kDa; LAP-2; HSP90N; LAP2; HSP90A; HSPCAL4; HSP89A; HSP86; HSP 86; HSPCA; Lipopolysaccharide-associated protein 2; HSPCAL1; LPS-associated protein 2; HSPN; Renal carcinoma antigen NY-REN-38; Heat shock protein HSP 90-alpha; Hsp89 |
Application Instructions
| Application Note | Western blot: use at 1 - 5 ug/ml. A band of ~90 kDa is detected. Immunoprecipitation: 5 ug on 20 ul Protein A - Sepharose + 100 ul sample. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| Positive Control | Heat-shocked HeLa cell lysate. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity chromatography |
| Buffer | PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSP90AA1 |
| Gene Full Name | heat shock protein 90kDa alpha (cytosolic), class A member 1 |
| Background | Hsp90 and the 94 kDa glucose-regulated protein, Grp94, are major molecular chapeones of the cytosol and endoplasmic reticulum. In mammalian cells, there are at least two Hsp90 isoforms, Hsp90α and Hsp90β, which are encoded by separate genes. All known members of the Hsp90 family are highly conserved, especially in the N-terminal and C-terminal regions. In the absence of stress, Hsp90 is an essential component of cellular processes such as hormone signaling and cell cycle control. Several regulatory proteins such as steroid receptors, cell cycle kinases and p53 have been identified as substrates of Hsp90. |
| Function | Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (By similarity). [UniProt] |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 85 kDa |
| PTM | ISGylated. S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. |
Clone References
A systems biology-based investigation into the therapeutic effects of Gansui Banxia Tang on reversing the imbalanced network of hepatocellular carcinoma.
WB, IHC-P / Mouse
LPS induces pp60c-src-mediated tyrosine phosphorylation of Hsp90 in lung vascular endothelial cells and mouse lung.
Regulation of mouse steroidogenesis by WHISTLE and JMJD1C through histone methylation balance.
WB, IP / Mouse
Hsp90 cleavage by an oxidative stress leads to its client proteins degradation and cancer cell death.
WB / Human
Characterization of a novel 23-kilodalton protein of unactive progesterone receptor complexes.
