ARG55956
anti-Hsp 60 antibody [LK1]
anti-Hsp 60 antibody [LK1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [LK1] recognizes Hsp 60 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Species Does Not React With | Bacteria |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | LK1 |
| Isotype | IgG1, kappa |
| Target Name | Hsp 60 |
| Antigen Species | Human |
| Immunogen | Recombinant Human Hsp60 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Heat shock protein 60; HuCHA60; 60 kDa heat shock protein, mitochondrial; GROEL; P60 lymphocyte protein; 60 kDa chaperonin; HSP-60; Mitochondrial matrix protein P1; HLD4; SPG13; HSP60; Chaperonin 60; Hsp60; CPN60; HSP65 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSPD1 |
| Gene Full Name | heat shock 60kDa protein 1 (chaperonin) |
| Background | This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010] |
| Function | Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt] |
| Cellular Localization | Mitochondria in cytoplasm |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody |
| Calculated MW | 61 kDa |
Images (4) Click the Picture to Zoom In
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ARG55956 anti-Hsp 60 antibody [LK1] WB image
Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG55956 anti-Hsp 60 antibody [LK1] at 0.4 µg/ml dilution.
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ARG55956 anti-Hsp 60 antibody [LK1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human breast carcinoma stained with ARG55956 anti-Hsp 60 antibody [LK1].
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ARG55956 anti-Hsp 60 antibody [LK1] ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55956 anti-Hsp 60 antibody [LK1] at 1:200 dilution. Left: primary antibody (green). Middle: DAPI (blue). Right: Merge.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG55956 anti-Hsp 60 antibody [LK1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded pancreas tissue stained with ARG55956 anti-Hsp 60 antibody [LK1].
Customer's Feedback
Excellent
Review for anti-Hsp 60 antibody [LK1]
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
Review for anti-Hsp 60 antibody [LK1]
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
Review for anti-Hsp 60 antibody [LK1]
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Good
Review for anti-Hsp 60 antibody [LK1]
Application：WB
Sample：293T, Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：0.4 µg/ml
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
