ARG54173
anti-Hsp 60 antibody
anti-Hsp 60 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody recognizes Hsp 60 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Target Name | Hsp 60 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human Hsp60 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Heat shock protein 60; HuCHA60; 60 kDa heat shock protein, mitochondrial; GROEL; P60 lymphocyte protein; 60 kDa chaperonin; HSP-60; Mitochondrial matrix protein P1; HLD4; SPG13; HSP60; Chaperonin 60; Hsp60; CPN60; HSP65 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 2.6 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSPD1 |
| Gene Full Name | heat shock 60kDa protein 1 (chaperonin) |
| Background | Implicated in mitochondrial protein import and macromolecular assembly.May facilitate the correct folding of imported proteins.May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. |
| Function | Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt] |
| Cellular Localization | Mitochondrion matrix. |
| Highlight | Related Antibody Duos and Panels: ARG30271 Mitochondrial Marker Antibody Panel (Cytochrome C, COX4, HSP60) Related products: Hsp60 antibodies; Hsp60 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody |
| Calculated MW | 61 kDa |
Images (4) Click the Picture to Zoom In
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ARG54173 anti-Hsp 60 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54173 anti-Hsp 60 antibody at 1:100 dilution. Left: primary antibody (green). Middle: DAPI (blue). Right: Merge.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG54173 anti-Hsp 60 antibody WB image
Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG54173 anti-Hsp 60 antibody at 1:1000 dilution.
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ARG54173 anti-Hsp 60 antibody WB image
Western blot: 15 µg of H1299, U87, HeLa, Jurkat and Raw264.7 cell lysates stained with ARG54173 anti-Hsp 60 antibody.
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ARG54173 anti-Hsp 60 antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54173 anti-Hsp 60 antibody.
Customer's Feedback
Excellent
Review for anti-Hsp 60 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
Review for anti-Hsp 60 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
Review for anti-Hsp 60 antibody
Application：WB
Sample：293T, Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Hsp 60 antibody
Application：WB
Sample：A: Mouse brain, B: Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
RSV replication is promoted by autophagy-mediated inhibition of apoptosis.
WB / Human
