ARG56939

anti-Hsp 40 antibody [k1C7]

anti-Hsp 40 antibody [k1C7] for Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [k1C7] recognizes Hsp 40
Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone k1C7
Isotype IgG2a, kappa
Target Name Hsp 40
Antigen Species Human
Immunogen Recombinant fragment around aa. 1-340 of Human Hsp40.
Conjugation Un-conjugated
Alternate Names Hsp40; HSP40; DnaJ protein homolog 1; hDj-1; HSPF1; Human DnaJ protein 1; Hdj1; RSPH16B; Heat shock 40 kDa protein 1; DnaJ homolog subfamily B member 1; Heat shock protein 40; Sis1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3337 Human DNAJB1

Swiss-port # P25685 Human DnaJ homolog subfamily B member 1

Gene Symbol DNAJB1
Gene Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Background This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Function Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. [UniProt]
Calculated MW 38 kDa

Images (2) Click the Picture to Zoom In

  • ARG56939 anti-Hsp 40 antibody [k1C7] WB image

    Western blot: 50 µg of Jurkat cell lysate stained with ARG56939 anti-Hsp 40 antibody [k1C7] at 1:1000.

  • ARG56939 anti-Hsp 40 antibody [k1C7] WB image

    Western blot: 40 µg of 1) 293T cell lysate, 2) HSP40 Transfected 293T cell lysates stained with ARG56939 anti-Hsp 40 antibody [k1C7] at 1:1000.