ARG54115
anti-Hsp 27 antibody
anti-Hsp 27 antibody for ICC/IF,Western blot and Human,Monkey
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes Hsp 27 |
|---|---|
| Tested Reactivity | Hu, Mk |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | Hsp 27 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human Hsp27 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | HSP 27; Heat shock 27 kDa protein; HMN2B; HS.76067; SRP27; HEL-S-102; HspB1; CMT2F; 28 kDa heat shock protein; HSP27; Heat shock protein beta-1; Hsp25; Estrogen-regulated 24 kDa protein; Stress-responsive protein 27; HSP28 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 27 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.2% Sodium azide, 50% Glycerol and 0.1%BSA |
| Preservative | 0.2% Sodium azide |
| Stabilizer | 50% Glycerol, 0.1%BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSPB1 |
| Gene Full Name | heat shock 27kDa protein 1 |
| Background | Involved in stress resistance and actin organization. |
| Function | Involved in stress resistance and actin organization. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 23 kDa |
| PTM | Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925). |
Images (2) Click the Picture to Zoom In
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ARG54115 anti-Hsp 27 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG54115 anti-Hsp 27 antibody at 1:300 dilution.
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ARG54115 anti-Hsp 27 antibody WB image
Western blot: A431 cell lysate stained with ARG54115 anti-Hsp 27 antibody at 1:1000 dilution.
