ARG10684
anti-Hsp 27 antibody
anti-Hsp 27 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Cow,Horse,Monkey,Pig
Overview
| Product Description | Chicken Polyclonal antibody recognizes Hsp 27 |
|---|---|
| Tested Reactivity | Hu, Cow, Hrs, Mk, Pig |
| Predict Reactivity | Chk |
| Species Does Not React With | Ms, Rat |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Target Name | Hsp 27 |
| Immunogen | Recombinant full length purified HSP27 from E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | HSP 27; Heat shock 27 kDa protein; HMN2B; HS.76067; SRP27; HEL-S-102; HspB1; CMT2F; 28 kDa heat shock protein; HSP27; Heat shock protein beta-1; Hsp25; Estrogen-regulated 24 kDa protein; Stress-responsive protein 27; HSP28 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSPB1 |
| Gene Full Name | heat shock 27kDa protein 1 |
| Background | The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008] |
| Function | Involved in stress resistance and actin organization. [UniProt] |
| Calculated MW | 23 kDa |
| PTM | Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925). |
Images (4) Click the Picture to Zoom In
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ARG10684 anti-Hsp 27 antibody ICC/IF image
Immunocytochemistry: HeLa cells stained with ARG10684 anti-Hsp 27 antibody (green), and co-stained with a monoclonal antibody to High mobility Group B protein 1 (HMGB1, red) and DNA (blue). ARG10684 reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins, while the HMGB1 antibody reveals strong nuclear staining which overlaps with the DNA staining.
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ARG10684 anti-Hsp 27 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG10684 anti-Hsp 27 antibody (green) at 1:1000 dilution and costained with Mouse mAb to Hsp 60 (red) at 1:5000 dilution. DAPI (blue) for nuclear staining.
The Hsp 27 antibody produces strong cytoplasmic staining, while the Hsp 60 antibody specifically labels mitochondria.
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ARG10684 anti-Hsp 27 antibody WB image
Western blot: HeLa cell crude extracts stained with ARG10684 anti-Hsp 27 antibody.
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ARG10684 anti-Hsp 27 antibody WB image
Western blot: NIH/3T3, C6, HeLa, HEK293, SH-SY5Y and COS-1 cell lysates stained with ARG10684 anti-Hsp 27 antibody (red) at 1:2000 dilution. The blot was simultaneously stained with Mouse mAb to beta tubulin (green) at 1:10000 dilution.
Note that the antibody does not recognize rodent Hsp 27.
