ARG64012
anti-Hsc 70 antibody
anti-Hsc 70 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Hsc 70 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody is expected to recognise isoform 1 (NP_006588.1) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Hsc 70 |
| Antigen Species | Human |
| Immunogen | C-EKLQGKINDEDKQK |
| Conjugation | Un-conjugated |
| Alternate Names | NIP71; HEL-33; HSPA10; LAP-1; HSC70; HSC71; LAP1; Lipopolysaccharide-associated protein 1; Heat shock 70 kDa protein 8; HSC54; HEL-S-72p; Heat shock cognate 71 kDa protein; HSP71; HSP73; LPS-associated protein 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 71 kDa |
| PTM | Acetylated. ISGylated. Trimethylation at Lys-561 reduces fibrillar SNCA binding. |
Images (3) Click the Picture to Zoom In
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ARG64012 anti-Hsc 70 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG64012 anti-Hsc 70 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized Goat IgG (green) at 10 µg/ml dilution.
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ARG64012 anti-Hsc 70 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64012 anti-Hsc 70 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64012 anti-Hsc 70 antibody WB image
Western blot: 35 µg of HepG2 and NIH/3T3 cell lysates (in RIPA buffer) stained with ARG64012 anti-Hsc 70 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
