ARG22206
anti-Hsc 70 antibody [1F2-H5]
anti-Hsc 70 antibody [1F2-H5] for ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [1F2-H5] recognizes Hsc 70 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, ICC/IF, IHC, IP, WB |
| Specificity | Detects ~73kDa. Does not cross react with HSP70. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1F2-H5 |
| Isotype | IgG2a, kappa |
| Target Name | Hsc 70 |
| Antigen Species | Human |
| Immunogen | Full length Human HSC70 |
| Conjugation | Un-conjugated |
| Alternate Names | NIP71; HEL-33; HSPA10; LAP-1; HSC70; HSC71; LAP1; Lipopolysaccharide-associated protein 1; Heat shock 70 kDa protein 8; HSC54; HEL-S-72p; Heat shock cognate 71 kDa protein; HSP71; HSP73; LPS-associated protein 1 |
Application Instructions
| Application Suggestion |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSPA8 |
| Gene Full Name | heat shock 70kDa protein 8 |
| Background | This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Function | Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP. [UniProt] |
| Cellular Localization | Cytoplasm, Melanosome |
| Calculated MW | 71 kDa |
| PTM | Acetylated. ISGylated. Trimethylation at Lys-561 reduces fibrillar SNCA binding. |
Images (3) Click the Picture to Zoom In
-
ARG22206 anti-Hsc 70 antibody [1F2-H5] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22206 anti-Hsc 70 antibody [1F2-H5] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
-
ARG22206 anti-Hsc 70 antibody [1F2-H5] WB image
Western blot: 15 µg of Human cell lysates stained with ARG22206 anti-Hsc 70 antibody [1F2-H5] at 1:1000 dilution (2 hours at RT).
-
ARG22206 anti-Hsc 70 antibody [1F2-H5] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22206 anti-Hsc 70 antibody [1F2-H5] (yellow) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
