ARG56027
anti-Histone antibody [1415-1]
anti-Histone antibody [1415-1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [1415-1] recognizes Histone |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1415-1 |
| Isotype | IgG2a, kappa |
| Target Name | Histone |
| Antigen Species | Human |
| Immunogen | Nuclei of Human leukemia biopsy cells. |
| Conjugation | Un-conjugated |
| Alternate Names | Histone H1.0; H1FV; H10; 0; Histone H1; Histone H1' |
Application Instructions
| Application Suggestion |
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| Application Note | Antigen retrieval for IHC-P: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | H1F0 |
| Gene Full Name | H1 histone family, member 0 |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008] |
| Function | Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt] |
| Cellular Localization | Nuclear |
| Calculated MW | 21 kDa |
| PTM | Phosphorylated on Ser-17 in RNA edited version. ADP-ribosylated on Ser-104 in response to DNA damage. |
Images (6) Click the Picture to Zoom In
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ARG56027 anti-Histone antibody [1415-1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human tonsil stained with ARG56027 anti-Histone antibody [1415-1].
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ARG56027 anti-Histone antibody [1415-1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human tonsil stained with ARG56027 anti-Histone antibody [1415-1].
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ARG56027 anti-Histone antibody [1415-1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human testicular carcinoma stained with ARG56027 anti-Histone antibody [1415-1].
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ARG56027 anti-Histone antibody [1415-1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human pancreas stained with ARG56027 anti-Histone antibody [1415-1].
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ARG56027 anti-Histone antibody [1415-1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human ovarian carcinoma stained with ARG56027 anti-Histone antibody [1415-1].
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ARG56027 anti-Histone antibody [1415-1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human angiosarcoma stained with ARG56027 anti-Histone antibody [1415-1].
