ARG45029
anti-Histone H4 monomethyl (Lys5) antibody [RM457]
anti-Histone H4 monomethyl (Lys5) antibody [RM457] for ELISA,Western blot and Human
Overview
| Product Description | Rabbit monoclonal antibody recognizes Histone H4 monomethyl (Lys5). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Specificity | This antibody reacts to Histone H4 monomethylated at Lysine 5 (K5me1). No cross reactivity with non-modified Lysine 5, or other monomethylations in histone H4. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | RM457 |
| Isotype | IgG |
| Target Name | Histone H4 |
| Antigen Species | Human |
| Immunogen | A monomethyl-peptide corresponding to monomethyl-Histone H4 (Lys5). |
| Conjugation | Un-conjugated |
| Alternate Names | H4C1; H4 Clustered Histone 1; HIST1H4A; H4FA; Histone Cluster 1 H4 Family Member A; H4 Histone Family, Member A; Histone Cluster 1, H4a; Histone 1, H4a; Histone H4; H4-16; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST2H4; HIST4H4; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4/A; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; H4/O; H4FO |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide |
| Preservative | 0.09% sodium azide |
| Stabilizer | 50% Glycerol, 1% BSA and 0.09% |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Gene Symbol | H4C1 |
|---|---|
| Gene Full Name | H4 Clustered Histone 1 |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [Uniprot] |
| Cellular Localization | Chromosome, Nucleosome core, Nucleus. [Uniprot] |
