ARG54774
anti-Histone H4 monomethyl (Lys20) antibody
anti-Histone H4 monomethyl (Lys20) antibody for Dot blot,ICC/IF,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Histone H4 monomethyl (Lys20) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | Dot, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Histone H4 |
| Antigen Species | Human |
| Immunogen | Synthetic methylated peptide around Lys20 of Human histone H4 (NP_003539.1) |
| Conjugation | Un-conjugated |
| Alternate Names | H4/p; Histone H4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HIST2H4A |
| Gene Full Name | histone cluster 2, H4a |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 11 kDa |
Images (2) Click the Picture to Zoom In
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ARG54774 anti-Histone H4 monomethyl (Lys20) antibody ICC/IF image
Immunofluorescence: 293T cells stained with ARG54774 anti-Histone H4 monomethyl (Lys20) antibody.
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ARG54774 anti-Histone H4 monomethyl (Lys20) antibody WB image
Western blot: HeLa cell lysate and Recombinant Histone H4 protein expressed in E. coli (negative control) stained with ARG54774 anti-Histone H4 monomethyl (Lys20) antibody.
